PlateDesigner was developed for basic science researchers and statisticians to define a rigorous experimental design prior to running a microplate assay. It is straightforward to use and highly customizable. One can control the position of technical replicates and addition of control samples, such as background, quality and intra-plate controls, and dilution samples needed to estimate standard curves. Importantly, samples are randomized so that experimental conditions are well-balanced and properly grouped within plates (eg., samples for repeated visits from same patients will always be in the same plate). Plates are also populated sequentially, so that most of the empty wells are put together on the last plate allowing for its use in the future experiments.
To use PlateDesigner, click here
PlateDesigner does not require any knowledge about randomization techniques or programming. Researchers just need to upload an excel-like comma separated (csv) file containing basic phenotypic information about the samples. Based on this, they will be prompted to make menu selections for their experimental setup. Outputs are available within seconds and include
1) a color-coded template (in pdf) for each plate with the experimental design to be followed by the technician,
2) a microplate reader friendly text file to be imported into the xPONENT (and other readers) software. PlateDesigner facilitates randomization, allowing seamless execution of well-designed, un-biased experiments.